Indian Society of Genetics & Plant Breeding

Genetic variation among Jatropha curcas L. using dominant molecular marker collected from different agro-climatic regions of India

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Genetic variability and relationship among 182 accessions
of Jatropha curcas collected from total 19 Indian states
were analysed based on random amplified polymorphic
DNA (RAPD) marker. A total of 30 primers were chosen
which produced 263 bands of which 254 were polymorphic
(96.82%) across the studied accessions.The polymorphic
information content (PIC) was highest for the primer Oligo
905 (0.901) and the lowest was OPAD 11 (0.620) with an
average of 0.791. Jaccard’s coefficient of similarity varied
from 0.31 to 0.98 indicates the high level of genetic variation
among the genotypes studied.The overall grouping pattern
of clustering corresponds well with the principal component
analysis (PCA), confirming patterns of genetic diversity
observed among the accessions.

Keywords: Jatropha curcas, genetic diversity, RAPD, molecular marker

Info

Year: 2015
Volume: 75
Issue: 2
Article DOI: 10.5958/0975-6906.2015.00043.7
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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Rekha Chaudhury and S. K. Malik info_circle

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