Indian Society of Genetics & Plant Breeding

Genetic similarity among five species of Saccharum based on isozyme and RAPD markers

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Genetic relationship among Saccharum species clones
was assessed using six isozyme systems and thirteen
RAPD primers. Multimeric isozyme profile and polymorphic
RAPD markers reflected the genetic complexity of the
genus. Clones could be distinguished from one another
only based on the esterase profile though species affinities
could not be detected using this system. Clonal distinction
and intraspecies similarities could be understood using
a combination of six isozyme systems studied. Low genetic
dissimilarity index within Saccharum and overlapping of
isozyme pattern of a few clones with other species were
suggestive of continuous variation across Saccharum
species. Species affinities as revealed through dendrogram
constructed based on Phylogenetic Analysis Using
Parsimony indicated closeness between S. barberi and S.
sinense and between S. officinarum and S. robustum
clones. Relationship between S. spontaneum with S. barberi
and S. sinense observed through RAPD analysis supported
S. spontaneum based origin of the species. The results
of isozyme and RAPD analyses were comparable and
agreed to the classically derived evolutionary affinities of
the genus
 

Keywords: Isozyme, RAPD, Saccharum species, genetic similarity, dendrogram

Info

Year: 2001
Volume: 61
Issue: 4
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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