Indian Society of Genetics & Plant Breeding

Genetic relationship among accessions of Stylosanthes hamata based on seed proteins profile

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For improvement of any plant character through
hybridization, it is necessary to understand the genetic
similarity/dissimilarity among the accessions. In the
present study an attempt was made to determine the
genetic relationship in 61 accessions of S. hamata using
seed-SDS-proteins. The genus Stylosanthes
(Fabaceae) consisting of approximately 40 diploid and
polyploid species has been classified on the basis of
morphological characteristics [1]. Five species of this
genus, namely Stylosanthes hamata, S. scabra, S.
viscosa, S. guianensis and S. humilis are widely used
as tropical forage legumes. Among these, S. scabra and
S. hamata are allotetraploids (2n = 4x = 40) and rest
three species are diploid (2n = 2x = 20). These five
species are predominantly self-pollinating with a low but
variable degree of outcrossing. Close relationship
between tetraploid S. hamata and S. humilis has been
reported by Stace and Cameron [2]. Results of seedprotein analysis [3], rhizobial affinities [4] and
morphological and agronomic characters [5] also yielded
similar observations. Introduction of a new diploid
species, i.e., S. seabrana, in 1998 from Australia was
visualized another potential species of stylo in India [6].
Among the species introduced in India from Australia
and South America, S. hamata has been most commonly
grown and naturalized but no emphasis has been given
on characterization of the species and accessions in
past. New accessions and species have been regularly
introduced from other countries to fortify the germplasm
holding as well as for their proper evaluation and
utilization. In the work reported here, the genetic
relationship among accessions of S. hamata has been
studied based on seed-sodium-dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE) protein
profile, and grouping based on un-weighted pair group
method with arithmetic mean algorithm (UPGMA)
analysis has been made. Such information would be
useful in systematic exploitation of the existing
collections and identifying accessions of high divergence
that could be used in future crossing programmes.

Keywords: N/A


Year: 2008
Volume: 68
Issue: 2
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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