Indian Society of Genetics & Plant Breeding

Genetic elimination of Kunitz trypsin inhibitors (KTI) from DS9712, an Indian soybean variety

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In this study, the null allele of Kunitz trypsin inhibitor i.e.
kti was transferred from PI542044, a germplasm line free
from KTI in to a popular Indian soybean variety DS9712
through marker assisted backcross breeding (MABB)
approach. Following foreground selection with 3 SSR
markers viz., Satt228, Satt429 and Satt409 that are linked
to kti, and background selection with 93 polymorphic SSR
markers in BC1F1 and BC2F1 generations, target plants
were selected that had 96-98% recovery of the recurrent
parent genome (RPG). In BC2F2 generation, plants
homozygous the for target allele (kti kti) were identified
and harvested individually. In BC2F3 generation, seed
proteins of the selected lines were extracted and analyzed
through native polyacrylamide gel electrophoresis (PAGE)
and confirmed absence of the KTI peptides. Four lines
were identified that were free from Kunitz trypsin inhibitor
but retained nearly all of the phenotypic features of
DS9712.This study exemplified successful elimination of
KTI from soybean seeds through MABB approach

Keywords: KTI, MABB, anti-nutritional factor, soybean, recombinant selection


Year: 2014
Volume: 74
Issue: Supplementry Issue
Article DOI: 10.5958/0975-6906.2014.00898.0
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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