Indian Society of Genetics & Plant Breeding

Genetic diversity studies of elite cotton germplasm lines using RAPD markers and morphological characteristics

Published on

Genetic diversity was studied in a set of 30 elite cotton
germplasm lines using RAPD markers and morphological
characteristics. These included 20 genotypes of G.
hirsutum, seven of G. arboreum, one genotype each of
G. herbaceum, G. thurberi and G. klostzschianum. For
RAPD analysis 32 random oligonucleotide primers were
used and only 25 showed amplification. A total of 108
bands amplified in 30 genotypes of which 4 were present
unifon-nly in all the 30 genotypes. One primer, Oligo
656, amplified an 1100bp G. arboreum specific band.
Total bands amplified for each primer ranged from 1 to
8, with an average of 4.4 fragments per primer. Nei and
Li' s similarity coefficient estimates ranged from 0.5 to
0.9. The wild species G. klostzschianum showed the least
similarity coefficient of 0.5 with all the lines of G. hirsutum,
G. arboreum and G. herbaceum as well as with another
wild species G. thurberi. Within G. hirsutum and G.
arboreum, the similarity coefficient ranged from 0.6 to
0.9. Clustering was done using the symmetric matrix of
similarity coefficients and cluster obtained based on
Unweighted Pair Group Method based on Arithmetic
Average (lTGMA). All the G. hirsutum genotypes except
NPl 83 group in one cluster. The G. arboreum genotypes
group in two clusters and the two wild species G. thurberi
and G. klostzschianum formed independent groups. At
80% similarity 15 of the 20 G. hirsutum genotypes grouped
in one cluster thereby revealing a very narrow genetic
base. More or less similar clusters were obtained based
on nine morphological characteristics measured on the
genotypes in the field experiments. Results reveal a
narrow genetic base within hirsutum and within arboreum

Keywords: Cotton, genetic similarity, RAPD markers


Year: 2003
Volume: 63
Issue: 1
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



Download PDF