Indian Society of Genetics & Plant Breeding

Genetic diversity and aggressiveness of different groups of Bipolaris sorokiniana isolates causing spot blotch disease in barley (Hordeum vulgare L.)

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Two hundred twenty five isolates of B. sorokiniana of barley were studied for their morpho-pathological  characterization and were grouped in to four categories (1) dull white to slight black, gel type cottony growth (DW) (2) white fluffy growth (WF), (3) suppressed white growth (SW) and (4) black fluffy growth (BF).The frequency of BF isolate was highest (39.6 %) whereas SW isolates displayed lowest frequency (7.1 %). The group IV (BF)   isolate was most aggressive. Sixty four purified isolates, sixteen from each of the four groups, were taken for RAPD variants analysis. Twenty RAPD primers were tested to detect the  variability among these four identified groups. A total of  204 bands were amplified with 100% polymorphism using 20 primers. Dendrogram based on molecular  polymorphism displayed considerable diversity within and resistance between groups of 64 isolates which displayed four  morpho-pathological groups into seven clusters. Specific DNA bands were identified for the selected isolates. The distinct markers may potentially be employed as genetic fingerprints for specific strain identification and classification in future. 

Keywords: Fungal DNA, RAPD markers, Bipolaris sorokiniana, spot blotch, isolate, barley


Year: 2012
Volume: 72
Issue: 1
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


R. Chand info_circle
A. K. Joshi info_circle

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