Indian Society of Genetics & Plant Breeding

Genetic diversity analysis in tuberose (Pollianthes tuberosa) genotypes through Randomly Amplified Polymorphic DNA

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The present investigation was undertaken for
characterizing tuberose genotypes using DNA marker
technology. Twenty tuberose genotypes comprising of
both single- and double-petal types collected from
different parts of India were selected for analysis. The DNA
extraction and RAPD conditions were standardized. For
RAPD analysis 20 ng DNA template, 2.5 mM MgCl2 and 1U
TaqDNA polymerase was found effective. Out of 80 random
decamer primers tested, 17 were selected based on high
level of polymorphism. On the basis of primer resolving
power and marker index, RAPD primers OPC-13 and OPD-
12 were identified as efficient primers for diversity analysis
of tuberose. A total of 157 RAPD bands were generated
by the 17 random decamer primers. The selected primers
proved effective for DNA profiling in addition to diversity
analysis. Genotypes Guwahati Double and Swarnrekha
showed good morphological similarity revealing high
similarity coefficient (0.90) suggesting them to be very
closely related. The RAPD analysis also confirmed their
relatedness as they grouped in the same cluster. The
suitability of this technique for genotyping and diversity
analysis was also established. Genotypes, Vaibhav and
Pune Single were found to have least pair-wise similarity
although they had a greater morphological similarity with
each other.
 

Keywords: Genetic diversity, Poliathes tuberosa, RAPD, tuberose, germplasm

Info

Year: 2010
Volume: 70
Issue: 2
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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K. V. Bhat info_circle
Anita Singh info_circle
Sanjay K. Singh info_circle

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