Indian Society of Genetics & Plant Breeding

Genetic analysis and tagging of gene controlling fruit tubercles and fruit ridgeness pattern in bitter gourd using SSR markers

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Thepresence of tubercles and pattern of ridges (continuous
vs discontinuous) are important traits of bitter gourd which
determine market price in India.The tuberculate fruits with
broken or discontinuous ridges have more unit price than
the non-tuberculate and continuous ridged fruits. In the
present study, two different botanical varieties of
Momordica charantia var. charantia (DBGS-2 and DBGS-
54) and
M. charantia var. muricata (DBGS-34) were used for
inheritance study and molecular tagging of fruit tubercles
and fruit ridgeness pattern. The findings revealed the
monogenic control for both the traits. The bi-parental
mapping populations (F
2) were developed from the crosses
DBGS-54×DBGS-34 and DBGS-2×DBGS-34 for tagging of
tuberculate (
Tb) and continues ridgeness (cr) locus, using
SSR markers. The single plant analysis of F
2 population
(derived from DBGS-54×DBGS-34) based on SSR markers
found McSSR-20 was linked to fruit tubercles gene (
Tb) at
12.50 cM and another SSR marker JY004 in the cross DBGS-
2×DBGS-34 was found linked with fruit ridgeness pattern
cr) gene at a distance of 19.6cM. It is the first report of SSR
markers linked to fruit tubercles and ridgeness which will
be helpful further in fine mapping of these traits with more
PCR based markers. These findings will help in
differenciating the genotypes with tubercles and pattern
of ridgeness at the early stage of the crop and can be used
in accelerating trait specific breeding programme in bitter

Keywords: Bitter gourd, fruit tubercle, fruit ridgeness, molecular marker, gene tagging


Year: 2019
Volume: 79
Issue: 4
Article DOI: 10.31742/IJGPB.79.4.14
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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