Indian Society of Genetics & Plant Breeding

Expression analysis of six chromatin and remodeling complex genes (SWR1) in chickpea in different tissues and during heat stress

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Nucleosome responds to heat stress through histone H2AZ
variants. Based on homology searches, we have identified
seven genes from the chickpea genome which are
homologous to chromatin remodeling complexes (SWR1)
in Arabidopsis. Chickpea homologs of PIE (Photoperiod
independent early flowering 1), ARP6 (Actin related protein),
two SEF (Serrated leaf and early flowering) and three H2AZs
(histone 2A variant-Z, a thermosensor in plants) genes were
analyzed for expression in chickpea under heat stress and
in five different tissues. Expression analysis of these genes
during heat stress at pod development stage showed only
PIE1 gene was upregulated and heat stress at seedling
stage resulted in significant down regulation. Tissue
specific expression analysis showed that the expression
of PIE1 and SEF was relatively higher in root, flower, pod
wall, and grain tissues as compared to that in shoots. Thus,
CarPIE1 gene might play an important role in chromatin
remodeling complexes during heat stress in chickpea and
all the three histone CarH2AZ variants can be good
candidate genes for the characterization of basic processes
of gene responsiveness in chickpea.

Keywords: SWR1 complex, heat stress response, phylogenetic analysis, pod development stage and gene expression


Year: 2016
Volume: 76
Issue: 1
Article DOI: 10.5958/0975-6906.2016.00007.9
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Prasanth Tej Kumar Jagannadham info_circle
Viswanathan Satheesh info_circle
Pradeep Kumar Jain info_circle
Ramamurthy Srinivasan info_circle

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