Indian Society of Genetics & Plant Breeding


Expression analysis of a MATE-type transporter gene of Arabidopsis and its orthologues in rice and chickpea under salt stress

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Salinity is one of the major abiotic stresses that adversely
affect the crop growth and productivity. Salt responsive
genes belonging to MATE efflux proteins reportedly play a
significant role imparting salt tolerance to plants. In the
present study, AT5G52050, a putative salt responsive gene
from Arabidopsis thaliana encoding MATE efflux family
protein which functions as an antiporter and its orthologues
in rice (LOC_Os02g45380) and chickpea (LOC101489496)
have been identified. The expression pattern of these genes
was validated by quantitative reverse transcription
polymerase chain reaction. In silico analysis of the upstream
promoter regions of these genes revealed the presence of
several conserved sequence motifs related to salt response
either in single or in multiple copies. A phylogenetic
analysis to understand the evolutionary relationship of
MATE family genes in Arabidopsis, rice and chickpea
revealed conservation of MATE family genes between dicots
and monocots. The genes identified in this study may serve
as promising candidates for further elucidation of the salt
tolerance mechanism in plants.

Keywords: Salinity, gene expression, arabidopsis, MATE-family genes


Year: 2015
Volume: 75
Issue: 4
Article DOI: 10.5958/0975-6906.2015.00076.0
Print ISSN: 0019-5200
Online ISSN: 0975-6906


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