Expression analysis and association of bulbing to FLOWERING LOCUS T (FT) gene in short day onion (Allium cepa L.)

Published on Mar 25, 2019 05:05 AM

The present study was carried out with the objective of
studying the expression of FLOWERING LOCUS T (FT)
genes responsible for bulbing in onion. Six AcFT genes
were used for expression studies at prebulbing (45 DAT),
bulbing (60 DAT) and post bulbing (75 DAT) stage. Two
varieties viz., Brown Spanish (long day) and Pusa Riddhi
(short day) were used. It was observed that out of the
reported six AcFT primers, four were able to amplify. Two
new primers exhibiting amplification of AcFT1 and AcFT6
are reported. In short day onion variety, Pusa Riddhi,
expression of five out of six genes studied (AcFT1, AcFT3,
AcFT4, AcFT5 and AcFT6) were highest at the bulbing stage
(60 DAT) suggesting their role in bulbing. In long day variety,
Brown Spanish, expression of all the six genes except
AcFT1 was very low at bulbing stage (60 DAT). This signifies
that specific photoperiod conditions for gene expression
were not achieved under Delhi conditions in long day onion.
More studies towards understanding of the genes need to
be addressed to understand the basic molecular process
of bulbing in short day onion.

Keywords: Bulbing, FLOWERING LOCUS T, short day onion, AcFT genes

Info

Year: 2019
Volume: 79
Issue: 1
Article DOI: 10.31742/IJGPB.79.1.10
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

Fornestar Lyngkhoi, Anil Khar, Manisha Mangal, Ambika B. Gaikwad and Nepolean Thirunavukkarasu

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