Evaluation of influence of different strains of Agrobacterium rhizogenes on efficiency of hairy root induction in Rauwolfia serpentina

Published on Jan 01, 2020 12:40 PM

An improved protocol was established for hairy root
induction in Rauwolfia serpentina using two strains of
Agrobacterium rhizogenes MTCC 532 and 2364. Hairy root
induction efficiency was standardized based on infection
time, co-cultivation period, OD600 and acetosyringone
concentration. Higher transformation efficiency was
established using MTCC 532 (31%) and MTCC 2364 (24%)
with 30 min infection time, 72 h co-cultivation period and
0.6 OD600. Transformation efficiency was further enhanced
to 55 % with 125 µM acetosyringone. MTCC 532 was proven
a better strain over the MTCC 2364 in all the tested factors.
Putative transgenic hairy roots were confirmed by
polymerase chain reaction using rolA specific primers.
Biomass of randomly selected 5 hairy root lines was also
significantly enhanced. No significant difference of growth
was recorded among the lines except line no 4. In the
present study, an enhanced system for Agrobacterium
rhizogenes-mediated hairy root culture was established
which offer an effective means to attain improved
transformation efficiency and ultimately beneficial for the
industrial scale in vitro production of the secondary
metabolite.  

Keywords: Hairy roots, Agrobacterium rhizogenes, Rauwolfia serpentina, MTCC 532, MTCC 2364

Info

Year: 2019
Volume: 79
Issue: 4
Article DOI: 10.31742/IJGPB.79.4.16
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

Pratima Bhagat, Sachin Kumar Verma, Amit Kumar Singh, Gajendra Kumar Aseri and Neeraj Khare info_circle

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