Evaluation of doubled haploid culture conditions and regeneration of an indica rice hybrid

Published on Jun 22, 2018 03:25 AM

A study was undertaken to develop an effective protocol to
generate doubled haploid plants from a proprietary Mahyco
hybrid MRP5401. A total of 20,300 anthers were plated on
four different DH media (basal N6 + phytohormones) and
obtained a callus induction success rate of 1.52%, of which
0.48% developed into green plants. A total of 232 lines were
obtained of which, 98 lines showed homozygosity in DH2-
3 generations. Well established DH lines from MRP5401 in
DH3 generation had early flowering and yield gain.

Keywords: Rice, doubled haploid (DH), anther culture, hybrid, callus induction, plant regeneration, genetic analysis

Info

Year: 2014
Volume: 74
Issue: 3
Article DOI: 10.5958/0975-6906.2014.00858.X
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

Anjanabha Bhattacharya, Venugopal Mikkilineni, Laxmikant Verma, Bhavesh Palan, Kishor Mali and Bhara

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