Indian Society of Genetics & Plant Breeding

Efficiency of RAPD markers in estimating the genetic relationship and development of DNA fingerprint in popular pigeonpea [Cajanus cajan (L.) Millsp.] cultivars

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In the present study, estimation of genetic diversity and
identification of pigeonpea cultivars and breeding lines
has been done using 121 RAPD primers, among which 20
were polymorphic. A total of 1193 bands were obtained
and 900 (75.4%) showed polymorphism between cultivars.
On an average 9.85 bands per primer were obtained.
Cluster analysis based on Jaccard’s similarity coefficient
using UPGMA grouped all the cultivars into three major
clusters. The clustering was strongly supported by high
bootstrap values.The arithmetic mean heterozygosity (Hav)
value, the average heterozygosity for polymorphic markers
(Hav) p value and marker index (MI) was found to be 0.499,
0.661 and 4.917 respectively. The genotype specific bands
can provide information to separate pigeonpea cultivars
among the 24 studied, which can definitely be of great
help in pigeonpea cultivar identification for cultivar-rightprotection.

Keywords: Pigeonpea, RAPD, molecular diversity, DNA fingerprinting


Year: 2012
Volume: 72
Issue: 3
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


B. George info_circle
A. K. Verma info_circle
Jyoti Kumari info_circle
Shiv Datt info_circle
G. Gupta and N. P. Singh info_circle

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