Indian Society of Genetics & Plant Breeding

Efficiency of double haploid production in wheat through wide hybridization and embryo rescue

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The effect of haploid induction in wheat F1s by Zea mays,
Imperata cylindrica and growth environments was
investigated. Doubled Haploid (DH) plant production via
maize and I. cylindrica technique from field grown LWH x
VL616 F1 plants was equally efficient. In second
environment, four F1 hybrids viz. LWH x VL616, HPW266 x
Yr15 (CN25087), HS490 x HI1563 and Local Red x WHD938
were grown in greenhouse conditions and pollinated with
maize. Efficiency of DH production was considerably high
from greenhouse grown F1s as compared to field grown
F1s. We also report an efficient protocol for DH production
in bread wheat.

Keywords: Wheat, doubled haploid, embryo rescue, Zea mays, I. cylindrica

Info

Year: 2017
Volume: 77
Issue: 3
Article DOI: 10.5958/0975-6906.2017.00059.1
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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S. C. Bhardwaj info_circle
O. P. Gangwar info_circle
Pramod Prasad info_circle
Ruchi Rathore info_circle

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