Indian Society of Genetics & Plant Breeding

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Effect of overexpression of Arabidopsis thaliana SHB1 and KLUH genes on seed weight and yield contributing traits in Indian mustard (Brassica juncea L. (Czern.))

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Seed size is a highly heritable trait in mustard and increasing
seed size could improve crop productivity. Here we report
the effect of overexpression of twoArabidopsis genesSHB1
and KLUH on seed weight and other yield contributing
traits inBrassica juncea. KLUH gene was expressed under
its own promoter, whereas the constitutive HPL promoter
droveSHB1. Transgenic status was confirmed through PCR,
RT-PCR and Southern analyses. The three SHB1 events
contained single copy of transgene, whereas 12 KLUH
events carried 1-4 copies of transgenes. All transgenics
were fertile and comparable to non-transgenic counterpart
for most of the agronomic traits in both T0 and T1
generations. Seed size and seed weight inSHB1 transgenics
were 30-40% higher than the control, whereas one KLUH
event showed significant increase in seed weight.
Constitutive overexpression of SHB1 in B. juncea had
undesirable pleiotropic effects that were comparable to
the Arabidopsis lines overexpressing IKU2.

Keywords: Indian mustard, seed weight, SHB1, KLUH, transformation

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Year: 2015
Volume: 75
Issue: 3
Article DOI: 10.5958/0975-6906.2015.00055.3
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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