Indian Society of Genetics & Plant Breeding

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Effect of growth regulators on androgenesis and regeneration in rice (Oryza sativa L.)

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Seven selected hybrids viz., CR 1009/HA 891037, CR
1009ITNAU 841434, ADT 40/AS95035, CO 43/ADT 43,
Improved White Ponni/CB 97033, Improved White Ponni
IIET 15341 and Improved White Ponni ICB(DH) 95298 were
anther cultured for the production of doubled haploid
lines. Maximum callus induction was observed in Improved
White Ponni ICB(DH) 95298 (32.67 per cent) and minimum
in CR 10091 HA 891037 (10.77 per cent). The cytokinin
treatment with 2.0mg/l BAP and 2.0mg/l GA3 accounted
for maximum regeneration. The frequency of callus induction was distinctly influenced by the genotype and growth
regulators. Callus induction occurred 20 to 25 days after
inoculation and maximum number of calli per tube were
formed between 30 to 75 days. The calli that emerged in
50 days had good competitive and morphogenetic potential
for green plantlet regeneration and those calli emerging
after 75 days had poor regeneration ability. Among the
seven hybrids cultured, green plant regeneration was
observed in only three hybrid combinations.
 

Keywords: Rice, anther culture, androgenesis

Info

Year: 2004
Volume: 64
Issue: 1
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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