Indian Society of Genetics & Plant Breeding

Effect of gamma ray irradiated pollen technique on seed development pattern in Citrus

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In modern fruit breeding, to reduce the breeding cycle,
induction of haploid plants through irradiated pollen
technique is of paramount importance. However, the major
drawback is the sensitivity of pollen to higher irradiation
doses as it induces abnormality in double fertilization
process. Hence, optimization of irradiation doses for
maximum recovery of healthy seed is very important.
Present work analyzed the seed developmental pattern of
Citrus grandis, pollinated with gamma irradiated pollen (100-
500 Gy) of
C. limon, C. limetta, and C. sinensis. Not a single
fruit was retained on the tree till maturity in
C. grandis × C.
crosses; however, in other two cross combinations,
fruits were harvested at maturity only up to 300 Gy irradiation
doses. Among the harvested fruit, normal seed decreased
gradually with increasing irradiation doses and less than 5
normal seeds per fruit were obtained at 300 Gy with
maximum in
C. grandis× C. limetta cross. However, abnormal
and empty number no. of seeds has increased significantly
with increasing irradiation doses. Developmental pattern
of embryo and endosperm within normal seeds was also
influenced significantly by irradiation treatment. At higher
doses, seeds number with both embryo and endosperm
was decreased gradually and at 300 Gy, it was reduced to
59.47% as compared to control.

Keywords: Abnormal seed, embryo, empty seed, endosperm, irradiation


Year: 2020
Volume: 80
Issue: 4
Article DOI: 10.31742/IJGPB.80.4.11
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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