Indian Society of Genetics & Plant Breeding

Effect of cytokinins and their concentration regimes on multiple shoot induction using cotyledonary node with cotyledons and embryo discs with half cotyledon explants in pigeonpea (Cajanus cajan L. Mi

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Pigeonpea (Cajanus cajan L. Milli sp.) belongs to family
Fabaceae, ranks sixth among legumes in production, which
is constrained by several biotic and abiotic stresses. Three
cytokinins viz., BAP, TDZ and zeatin and their concentration
regimes were tested for multiple shoot bud induction in
ICPL87119 and BSMR736 pigeonpea genotypes.
Fortification of growth medium with 2.0 mg/l BAP, 4.0 mg/
l TDZ and 2.0 mg/l zeatin, separately, could induce maximum
number of shoot buds, 53.7, 46.1 and 40.9 respectively, per
explants in both, cotyledonary node with cotyledons and
embryo discs with half cotyledon explants. Any further
increase in tested cytokinin concentration levels imparted
reduction in multiple shoot bud induction. There was no
significant effect of MS nutrient strength on root induction,
MS with 0.5 mg/l IBA was most suitable for healthy and
number of root induction (4.8±0.7). Reported in vitro method
of plantlet regeneration may be exploited for genetic
engineering programs for multiple stress tolerance in

Keywords: Cytokinin, IBA, pigeonpea, induction, regeneration, plantlet


Year: 2014
Volume: 74
Issue: 3
Article DOI: 10.5958/0975-6906.2014.00851.7
Print ISSN: 0019-5200
Online ISSN: 0975-6906


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