Indian Society of Genetics & Plant Breeding
Anthers from two cultivars of barley (Hordeum vulgare 1.), Chemigovski 5and Oneprovski
435, were cultured on N6 liquid and semisolid agar media with three different levels of
2,4-0 (2.0, 4.0 and 6.0 mgllitre) and 0.5 mgllitre kinetin. Significant results were obtained
for androgenic calli and embryoid production with increased 2,4-0 level, especially in
liquid medium preconditioned with isolated earheads. As a result, numberof plantlets was
also high although not much difference was observed among media supplemented with
three levels of 2,4-0 for the regeneration of green plantlets. Increased 2,4-0 produced
more androgenic calli than embryoids. The plantlets regenerated were mostly
dihaploids (2n = 2x = 14) and ranged from 65.7% to 84.6% for both the cultivars.
Keywords: Hordeum Vulgare, 2,4-0, androgenic calli, embryoids, dihaploids.
Year: 1991
Volume: 51
Issue: 1
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906
SHARAD TIWARI AND I. RAHIMBAEV info_circle
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