Indian Society of Genetics & Plant Breeding

Distribution of rDNA loci and genome differentiation in tetraploid Cynodon

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The 45S rDNA and 5S rDNA loci encodes for ribosomal
RNA. Mapping of rDNA loci has not been reported in
Cynodon. We used FISH technique to locate the loci coding
for rDNA. Our results showed that the centromeres of three
chromosomes had signals of different intensities in the
tetraploid bermudagrass line C121. The tetraploid line, C299,
had four signals at the centromeres of four chromosomes,
with two being strong and two relatively weaker. We found
three signals from C121 and four from line C299 about the
5S rDNA sequence. The rDNA signal numbers and sizes
indicated existence of genome differentiation in
bermudagrass. Combined results of staining pollen mother
cells at meiosis indicated that tetraploid bermudagrass has
at least two genome types

Keywords: Cynodon, tetraploids, rDNA, FISH, genome differentiation


Year: 2013
Volume: 73
Issue: 4
Article DOI: 10.5958/j.0975-6906.73.4.071
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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