Indian Society of Genetics & Plant Breeding

Important

Distribution of alleles of grain quality genes in Indian bread wheat varieties

Published on

The prevalence of puroindoline hardness mutations
(alleles) among Indian wheat varieties, germplasm lines,
Triticum spelta and synthetic derivative accessions was
surveyed in the present study. A total of 356 wheat
genotypes were examined. While most T. spelta accessions
had soft grains, very few among the rest of the lines were
soft and possessed wild- type alleles of the two
puroindoline genes. The majority were, however, uniformly
hard. The null mutation of Pina and the wild type Pinb allele
(Pina-D1b/Pinb-D1a) was the most frequent combination
in the Indian wheat germplasm. Out of the 356 lines
examined, only 42 possessed the Pinb-D1b allele
indicating gly46serine mutation in Pinb. One landrace
MPG33 possessed the Pinb-D1e allele as found by
sequencing the 447 bp product obtained with Pinb-D1
primer in this line. Distribution of the alleles of the serpin
(Srp5B) gene was also studied in 90 Indian wheat cultivars
released during 1985-2010. Only 11 of the 90 genotypes
possessed the b-type allele indicating the prevalence of
the desirable a-type allele in the Indian wheat varieties.
The role of the alleles of puroindoline and serpins genes
in end-use quality of wheat is discussed

Keywords: PINA, PINB, kernel texture, PinaD1, PinbD1, serpins, Srp5B, flour yield

Info

Year: 2012
Volume: 72
Issue: 2
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

info_circle
Indu Ravi info_circle
Mamata Yadav info_circle
P. C. Misra info_circle

Download PDF