Indian Society of Genetics & Plant Breeding


Distribution of alleles of grain quality genes in Indian bread wheat varieties

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The prevalence of puroindoline hardness mutations
(alleles) among Indian wheat varieties, germplasm lines,
Triticum spelta and synthetic derivative accessions was
surveyed in the present study. A total of 356 wheat
genotypes were examined. While most T. spelta accessions
had soft grains, very few among the rest of the lines were
soft and possessed wild- type alleles of the two
puroindoline genes. The majority were, however, uniformly
hard. The null mutation of Pina and the wild type Pinb allele
(Pina-D1b/Pinb-D1a) was the most frequent combination
in the Indian wheat germplasm. Out of the 356 lines
examined, only 42 possessed the Pinb-D1b allele
indicating gly46serine mutation in Pinb. One landrace
MPG33 possessed the Pinb-D1e allele as found by
sequencing the 447 bp product obtained with Pinb-D1
primer in this line. Distribution of the alleles of the serpin
(Srp5B) gene was also studied in 90 Indian wheat cultivars
released during 1985-2010. Only 11 of the 90 genotypes
possessed the b-type allele indicating the prevalence of
the desirable a-type allele in the Indian wheat varieties.
The role of the alleles of puroindoline and serpins genes
in end-use quality of wheat is discussed

Keywords: PINA, PINB, kernel texture, PinaD1, PinbD1, serpins, Srp5B, flour yield


Year: 2012
Volume: 72
Issue: 2
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Indu Ravi info_circle
Mamata Yadav info_circle
P. C. Misra info_circle

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