Differential effect of immature embryo’s age and genotypes on embryogenic type II callus production and whole plant regeneration in tropical maize inbred lines (Zea mays L.)

Published on Jun 21, 2018 03:14 AM

The study was undertaken to elucidate the effect of
genotypes and age of immature embryo on callus induction,
embryogenic type II calli production and regeneration from
immature maize embryos. The immature embryos were
excised from a selfed ear of ten elite Indian maize inbred
lines on 6, 12 and 18 days after pollination. The mean callus
induction, embryogenic type II calli production, and
regeneration was highest across all the genotypes in 12
days old immature embryos and were 44.25, 26.12, and 11.20
per cent respectively. The significant differences were
observed with respect to callus induction, embryogenic
type II calli production and regeneration capacity between
genotypes. The highest regeneration capacity was observed
by immature embryo at 12 days after pollination (DAP) of
genotype HKI1105 and CM300.
 

Keywords: Explants, embryogenic type II callus, genotype, maize and regeneration

Info

Year: 2014
Volume: 74
Issue: 3
Article DOI: 10.5958/0975-6906.2014.00849.9
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

Alok Abhishek, Chikkappa G. Karjagi,Meenakshi Bhardwaj,Pradyumn Kumar, Sain Dass and R. Sai Kumar info_circle
Ravindra Nath info_circle
W. Ramteke info_circle

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