Indian Society of Genetics & Plant Breeding

Development of tobacco (Nicotiana tabacum L.) doubled haploid lines resistant to potato virus Y 0

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Viral diseases such as Potato Virus Y (PVY) in tobacco plants
can cause a lot of damage annually. In order to obtain PVY
resistant doubled haploids (DH) of Nicotiana tabacum with
desirable traits. Two varieties of tobacco VAM resistant to
PVY and K326 of recognized high quality of leaves were
crossed. The F1 hybrids were grown in pots and the anthers
were cultured on PGR-free MS medium. After production
of somatic embryos, colchicine treatment (0.4%) was applied
for 8 hours. Thereafter, 184 plantlets were transferred to
greenhouse and then moved to the field and self-pollinated.
The derivatives of colchicine treated and fertile plants were
inoculated with PVY0. DAS-ELISA technique and rating of
symptoms were used to select resistant plants. From a
total of seventeen DH, seven plants were selected as pure
lines and evaluated under field condition. The DH lines
showed significant differences between them for
morphological and chemical traits. Some of the DHs had
longer and wider leaves than K326 cultivar as control. The
content of sugars was not affected by genotype. Finally,
three doubled haploid lines (TDH-03, TDH-09, TDH-11) with
desirable characteristics were selected as PVY resistance.

Keywords: Tobacco breeding, doubled haploid, potato virus Y, ELISA, GGE biplot


Year: 2016
Volume: 76
Issue: 3
Article DOI: 10.5958/0975-6906.2016.00050.X
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Azimeh Hosseini info_circle
Jahad Soorni info_circle
Anna Trojak-Goluch info_circle

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