Indian Society of Genetics & Plant Breeding

Development of SCAR marker for screening Sigatoka-leafspot resistance in banana genotypes

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Sigatoka leaf spot is a major fungal disease of banana
worldwide, particularly, in pacific island, from where this
pathogen originated. Not so many resistant genotypes are
reported against this. Initially, RAPD fragments correlating
with lower percentage of disease index (PDI) were identified
involving fifteen banana cultivars collected from diverse
zone of West Bengal. Four correlating RAPD fragments
from each two independent genotypes were cloned,
sequenced and submitted into GenBank. It had been
observed that sequence information was dissimilar even
though same RAPD primer amplified equal sized amplicon
from two different varieties. Even fragment from two
different clones of a variety, was also dissimilar. Based on
sequence information, eight pairs of SCAR primers were
designed. Only one SCAR primer based on the sequence
of OPA13640 from Kanthali Clone1 showed reproducible
dominant banding pattern like RAPD. Identified SCAR
marker when validated among additional 26 diverse
genotypes, collected across India, showed strong
correlation with the Sigatoka resistance lines. SCAR
markers identified and validated in this study will be useful
in screening Sigatoka resistance lines in future banana
breeding program.

Keywords: Banana, Sigatoka disease, SCAR, Kanthali clone 1


Year: 2016
Volume: 76
Issue: 1
Article DOI: 10.5958/0975-6906.2016.00010.9
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Subhasish Mondal info_circle
Dilip K. Mishra info_circle
Somnath Bhattacharyya info_circle

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