Indian Society of Genetics & Plant Breeding

Development of microsatellite markers in finger millet (Eleusine coracana Geartn.)

Published on

In the present study, for the first time an attempt was made
to isolate microsatellites in finger millet using pre-cloning
enrichment (selective hybridization) strategy which resulted
in isolation of very good number of micro satellites
(323).While developing the microsatellite-enriched library
for finger millet, more than 2200 colonies were screened,
and 1021 recombinant clones ranging from 500-1000bp were
selected for further sequencing. Sequence analysis revealed
that only 645 clones (63%) contained microsatellite motif
regions. Monorepeats C and T were abundant, whereas,
AG and CT class of dinucleotide repeats accounted about
21% and 30% respectively. Apart from the abundant, mono,
di-nucleotide and tri repeats, about 58 tetranucleotide and
62 penta repeats were also isolated.

Keywords: SSR, finger millet, enrichment, microsatellites, ragi

Info

Year: 2021
Volume: 81
Issue: 3
Article DOI: 10.31742/IJGPB.81.3.14
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

info_circle

Download PDF