Indian Society of Genetics & Plant Breeding

Development of highly polymorphic SSR markers for chickpea (Cicer arietinum L.) and their use in parental polymorphism

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Simple sequence repeat (SSR) or microsatellite marker
is currently the most preferred molecular marker
system owing to their highly desirable properties
abundance, hyper-variability, and suitability for highthroughput analysis. Development of SSR markers
using molecular methods is time consuming, laborious,
and expensive. Use of computational approaches to
mine ever-increasing sequences such as expressed
sequence tags (ESTs) and genomic DNA sequences
available in public databases permits rapid and
economical discovery of SSRs. Because the number
of SSR markers currently available in chickpea is very
limited, the aim of this study was to develop and
characterize more SSR markers. Twenty one hundred
DNA sequences of chickpea were searched for SSRs
and analyzed for the design of PCR primers amplifying
the SSR reach regions. Di-nucleotide repeats were
found to be the most abundant followed by tri- or mononucleotide repeats. The motifs AIT,
CAlTA, and CAAlTCT/AGAlCAGrrTG/ATT were the
predominant mono-, di-, and tri-nucleotide SSRs,
respectively. A subset of 64 primer pairs flanking SSR
loci was used for screening polymorphism between
two chickpea cultivars BG 256 and WR 315, which are
parents of a
Fusarium wilt mapping populations. Of
them, 45 SSR markers (70.3%) were polymorphic
between these two parents

Keywords: Microsatellites, ESTs, polymorphism, chickpea


Year: 2007
Volume: 67
Issue: 4
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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