Indian Society of Genetics & Plant Breeding

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Development of high throughput screening protocol and identification of novel sources of resistance against bakanae disease in rice (Oryza sativa L.)

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Bakanae or foot rot disease caused by Fusarium fujikuroi
(teleomorph: Gibberella fujikuroi, Sawada,Wollenweber) is
emerging as a serious disease of rice. A simple, reliable and
high-throughput method for screening the disease would
enable rapid screening of germplasm aimed at identifying
resistance sources, mapping QTLs/genes and developing
resistant rice cultivars. In the present study, a highthroughput, reliable bioassay to screen rice germplasm for
resistance to bakanae disease was developed and
compared with the conventional screening technique. This
technique involves soaking of rice seeds in fungal spore
suspension (1.0x106 spores ml–1) for 24 hours at room
temperature. Seedling growth at 30°/25° (±3)°C day/night
temperature and 60/80(±10)% day/night relative humidity in
glasshouse gave the best results. The new protocol
described here produces consistent and reproducible
bakanae disease symptoms and enables screening of
hundreds of rice germplasm within 15 days without any
loss of precision in screening of rice genotypes against
bakanae disease.The resistant and susceptible genotypes
can be used for developing mapping population and
identification of QTLs/genes conferring resistance to
bakanae disease.
 

Keywords: B. M. Bashyal

Info

Year: 2014
Volume: 74
Issue: 4
Article DOI: 10.5958/0975-6906.2014.00864.5
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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H. Rajashekara info_circle
B. M. Bashyal,N. K. Singh info_circle

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