Indian Society of Genetics & Plant Breeding

Development of EST-derived microsatellite markers in mungbean [Vigna radiata (L.) Wilczek] and their transferability to other Vigna species

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Twenty microsatellite markers were developed from
mungbean [Vigna radiata (L.) Wilczek] EST sequences
available in public database. Out of these, ten
microsatellite markers (50%) were found to be polymorphic
when screened on 22 genotypes. The number of alleles
ranged from two to three with an average of 2.3 alleles
per locus. Polymorphic information content of the
microsatellite markers ranged from 0.09 to 0.59 with an
average of 0.31. When these microsatellite markers were
screened on eight other Vigna species, they showed high
transferability (90%) to other Vigna species. These ESTderived microsatellite markers may be useful for linkage
mapping, gene tagging and genetic diversity analysis in
mungbean and other related Vigna species.

Keywords: Microsatellite markers, polymorphism, mungbean, allele, Vigna species

Info

Year: 2012
Volume: 72
Issue: 4
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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