Indian Society of Genetics & Plant Breeding

Development of an ISSR-derived SCAR marker linked to apospory in buffel grass (Cenchrus ciliaris L.)

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Cenchrus ciliaris (buffel grass) is a valuable forage grass,
reproducing through aposporous apomixis. Inter Simple
Sequence Repeat (ISSR) markers converted to SCAR marker
linked to apospory in this crop is reported. Out of twentyfive
ISSR primers tested in DNA bulks from apomictic and
sexual progenies, five primers detected polymorphism.The
1.1 kb amplicon derived from the primer ISSR-856 showed
co-segregation with apomictic mode of reproduction.
Accordingly, a pair of SCAR (Sequence Characterized
Amplified Region) primers were designed which also
generated a 1.1kb amplicon (Apo-856) only in the apomictic
plants. Importance of such molecular markers in genetics
and breeding of C. ciliaris is discussed.

Keywords: Apomixis, Cenchrus ciliaris, molecular marker, ISSR, SCAR


Year: 2015
Volume: 75
Issue: 2
Article DOI: 10.5958/0975-6906.2015.00044.9
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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