Indian Society of Genetics & Plant Breeding

DETECTION OF PROTEIN MARKERS FOR IDENTIFICATION OF RICE GENOTYPES RESISTANT TO GREEN LEAFHOPPER

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SDS-PAGE of total seed proteins of 11 rice genotypes resistant to green leafhopper
(GLH) and a susceptible variety, Taichung (Native) T(N) 1 facilitated scoring of 11
distinct polypeptide bands of heterogeneous molecular weights ranging from 84.1 to
24.0 kD. Seven bands exhibited polymorphism with regard to presence/absence or
molecular weight. Two bands (46.8 and 42.7 kD) were present only in the susceptible
variety and are potentially reliable markers in screening for GLH resistance. Five bands
of 53.7, 38.0, 33.9, 28.2 and 24.0 kD were polymorphic for presence/absence or molecular
weight even within the resistant genotypes. These polymorphic bands can be used as
markers in breeding for GLH resistance as well as for detection of identification of
unknown resistance genes and understanding the genetics of resistance.
 

Keywords: Rice, Green Leafhopper, Resistance, Protein Markers, Marker Assisted Breeding

Info

Year: 1999
Volume: 59
Issue: 4
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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