Indian Society of Genetics & Plant Breeding

Detection of genotype specific fingerprints and assessment of genetic diversity in elite genetic stocks of tomato (Solanum lycopersicum L.) using RAPD primers and agronomic traits

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Genotype specific DNA fingerprints and morphological
traits for genetic stocks of crop plants are pre-requisite
for germplasm registration with the competent authority
and for granting Plant Breeders’ Rights. Eight
morphological and three biochemical markers, and 18
RAPD primers were employed to estimate genetic diversity
and to characterize 30 genetic stocks of tomato
possessing special attributes. The analysis based on field
observations, biochemical constituents and RAPD primers
revealed wide genetic diversity in the germplasm
evaluated. The RAPD primers generated 192 bands of
which, 151 (78.6%) were polymorphic. The polymorphic
information content (PIC) values for the 18 primers ranged
from 0.76 in OPB-154 to 0.97 in S-1113. The similarity coefficient analysis revealed two clusters; the first cluster
comprised of only four genotypes and the second major
cluster comprised of 26 genotypes which could further
be classified in six sub-clusters. The RAPD analysis proved
helpful for estimating the magnitude of genetic diversity,
establishing genetic relatedness among genetic stocks
and for developing unique fingerprints of 21 out of 30
genetic stocks evaluated.

Keywords: Tomato, molecular markers, RAPDs, fingerprinting, genetic diversity


Year: 2009
Volume: 69
Issue: 1
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Sawtantar Singh info_circle
Kuldeep Singh info_circle

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