Indian Society of Genetics & Plant Breeding

Department of Genetics & Plant Breeding, G.B. Pant Univ. of Agric. & Tech. Pantnagar, Distt.U.S. Nagar 263 145

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Twenty six varieties of forage sorghum which included 20
released or notified and 6 indigenous local varieties were
characterized with 40 morphological DUS descriptors as
prescribed by DUS guidelines of PPV &FR Authority and
ICAR. RAPD and ISSR markers were also studied to
complement the morphological DUS descriptors for
establishing distinctiveness of a variety. When all the 33
morphological descriptors of PPV & FR Authority and 7
morphological descriptors of ICAR were studied distinct
morphological profiles could be obtained only for 11 out
of a total of 26 varieties. Thirteen primers out of a total of
14 RAPD primers were able to establish unique molecular
identification profiles (MIPs) for a total of 14 varieties.
Fourteen primers out of a total of 20 ISSR primers were
able to establish distinctiveness of 19 varieties by the
amplification of different genotype specific bands in these
varieties. Both the molecular markers revealed a very high
level of polymorphism, enabling genetic discrimination of
the varieties analyzed by using 121 informative RAPD and
178 ISSR bands. UPGMA cluster analysis of both the
markers could distinguish all the twenty six varieties.
Higher Mean polymorphic information content (PIC),
average expected gene diversity, average resolving power
(Rp) and diversity index (DI) were higher for ISSR marker
as compared to RAPD one which reflected that ISSR
marker is more efficient tool to establish distinctiveness
amongst the present set of experimental material. Out of
a total of 26 varieties unique identification profiles were
developed for 25 varieties by a combination of
morphological DUS descriptors and both the RAPD and
ISSR markers. However, the variety Pusa Chari 121 was
not delineated by any of the morphological and molecular
markers. Thus from the present study it could be
concluded that in situations where the morphophysiological DUS descriptors are not able to establish
distinctiveness of a variety then unique molecular fingerprints generated by molecular markers may be used
as additional or complement descriptors for resolving
distinctiveness of varieties.

Keywords: DUS, RAPD, ISSR, morphological descriptors


Year: 2011
Volume: 71
Issue: 1
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


P. K. Shrotria, Ravindra Singh, H. S. Chawla info_circle

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