Indian Society of Genetics & Plant Breeding

Cryoconservation of some wild species of Musa L.

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The present work was undertaken to devise suitable
medium for induction of proliferating meristems in five
wild species of Musa (M. acuminata, M. balbisiana, M.
basjo, M. jackeyi and M. textilis) and their subsequent
cryopreservation by modification of the standard droplet
vitrification protocol. It was found that BAP (100 ìM) alone
was not sufficient to induce high proliferation rates and
inclusion of TDZ (1-10 ìM) was essential. The duration of
pre-growth desiccation (9-14 d) and dehydration with
cryoprotectant (PVS2, 90-120 min) varied with each
species.The optimized protocol for each species yielded
46-58% shoot recovery after cryopreservation. In terms
of ease of explant generation, percentage shoot recovery
and plantlet formation, M. jackeyi > M. basjoo > M. textilis>
M. acuminata>M. balbisiana.

Keywords: Crop wild relatives, cryopreservation, Musa, vitrification


Year: 2014
Volume: 74
Issue: Supplementry Issue
Article DOI: 10.5958/0975-6906.2014.00907.9
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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