Indian Society of Genetics & Plant Breeding

Comparison of DNA profiles of traditional and modern bread wheat varieties of India

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The present investigation was undertaken to evaluate and
compare four pre green revolution indigenous varieties,
C306, C518, C591 and C273 and three elite wheat lines
PBW343, PBW534 and PBW550 of recent origin with
microsatellite markers. A total of 315 SSR primers yielded
185 polymorphic SSR loci and 460 alleles. Twenty five
unique/rare alleles in C varieties can be potentially useful in
breeding programmes. Genetic diversity was assessed by
clustering analysis in which a Dice Similarity Matrix was
generated using the SSR data and the dendrogram was
constructed using unweighted neighbour joining method
with 1000 bootstrap by Darwin5. The lines diverged into
two groups, one comprising C varieties and the second
elite wheat lines. The cluster analysis thereby revealing the
possibility of transferring useful variability from the old
varieties into new elite wheat cultivars.

Keywords: Wheat, landraces, C varieties, SSR, diversity analysis


Year: 2014
Volume: 74
Issue: 3
Article DOI: 10.5958/0975-6906.2014.00855.4
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Navtej S. Bains info_circle

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