Indian Society of Genetics & Plant Breeding

Comparative evaluation of genetic relationships among ber (Ziziphus sp.) genotypes using RAPD and ISSR markers

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Genetic diversity among 48 ber genotypes from different
eco-geographical regions of India was investigated using
random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers. Forty-six RAPD
and 18 ISSR primers generated a total of 368 and 167
amplification products of which 271 (74.15%) and 152
(89.94%) were polymorphic, respectively. The average
numbers of polymorphic fragments were 5.89 (RAPD) and
8.44 (ISSR) per primer with number of amplified bands
ranging between 2 to 17 and 4 to 12 for RAPD and ISSR
respectively. RAPD primer OPD-3 (9.00 Rp and 0.29 PIC)
and ISSR primer UBC-880 (5.63 Rp and 0.29 PIC) were the
most informative primers. Out of the 18 ISSR primers 14
were anchored, of which 12 were 3’-anchored and 2 were
5’-anchored. Fourteen ISSR primers produced distinct
bands contained dinucleotide repeats. Primers based on
(AC)n and (AG)n repeats produced the most polymorphic
bands. Based on mantel matrix correlation between two
marker systems it is suggested that the two markers
amplify different genomic regions. The genetic similarity
among 48 ber genotypes ranged from 47.62% (Thornless
and Seov) to 88.97% (Narma and Banarsi Karaka) that
suggested a wide genetic base for the ber germplasm
collection. The study suggests superiority of ISSR marker
system over RAPD for studying genetic diversity in ber.

Keywords: Genetic diversity, Ziziphus, ber, RAPD, ISSR


Year: 2009
Volume: 69
Issue: 1
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Rakesh Singh info_circle
N. K. Singh info_circle

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