Indian Society of Genetics & Plant Breeding

Comparative assessment of genetic purity of cotton hybrids by GOT and RAPD markers

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In the present investigation, a comparative study was made
between gro-out test (GOT) and molecular markers in
assessing genetic purity in the seeds of hybrid cotton. In
GOT, the genetic purity was detected based on color of the
anthers, while in molecular studies 20 RAPD markers were
used for the same purpose. The genetic purity in the seeds
of hybrid Vishwanath ranged from 92.9 to 95.1%, while the
same through RAPD markers (OPA-07) ranged from 94 to
96%. Results obtained in seeds lot of hybrid DCH-32 and
Varalaxmi were similar. The results of GOT and RAPD
markers were comparable. RAPD can be an efficient
replacement for otherwise tedious and time consuming
GOT tests.

Keywords: Genetic purity, grow-out test, hybrid, cotton, RAPD


Year: 2014
Volume: 74
Issue: 1
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


S. S. Gaurav info_circle
Akshay Talukdar info_circle

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