Indian Society of Genetics & Plant Breeding
Twelve field bean (Lablab purpureus) genotypes - nine
local collections, one check variety of filed bean and one
lima bean local type - were selected for the study. The data
on 23 quantitative traits revealed significant differences
among selected genotypes for many traits. The cluster
analysis of genotypes using 23 phenotypic traits revealed
3 clusters. Lima bean formed a separate cluster distinct
from field bean genotypes. The pendal and non-pendal
(both determinate and semi determinate) types formed two
separate clusters within field bean genotypes. The RAPD
analysis of 12 genotypes with 20 random decamer primers
produced 200 fragments of which 178 were polymorphic.
The diversity observed at molecular level was low. The
dissimilarity coefficient ranged from 3.23 percent to 11.66
percent among field bean genotypes. Limabean genotype
formed a distinct cluster with dissimilarity coefficient
ranging from 65.65 to 67.7%. A comparison was made
between genetic diversity at phenotypic and molecular
level. The genotypes exhibited limited diversity at
molecular level and higher diversity at phenotypic level.
There was no concurrence between phenotypic and
molecular diversity and clustering among the genotypes.
Therefore, the degree of genetic diversity appeared to be
overestimated if based solely on phenotypic variation in
field bean.
Keywords: Genetic diversity, RAPD markers, Field bean, Phenotypic variation Introduction
Year: 2009
Volume: 69
Issue: 3
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906
B. N. Savitha,R. L. Ravikumar info_circle
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