Indian Society of Genetics & Plant Breeding

Cloning and expression analysis of a novel Glutathione S-transferase gene, MsGST, from alfalfa (Medicago sativa)

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Glutathione S-transferases are important enzymes in
protecting cells by scavenging reactive oxygen species
induced by various stresses. In this study, a novel GST
gene, MsGST (KM044312), was cloned and characterized
from alfalfa. The open reading frame of MsGST contains
660bp nucleotides, encoding 219 amino acid residues.
Amino acid sequence alignment indicated that the deduced
MsGST protein was highly homologous to other plant tau
class GST sequences. According to amino acid phylogenetic
analysis, the MsGST gene was clustered into the same
branch with other legume plants. Real-time quantitative
PCR (qRT-PCR) revealed that the expression levels of
MsGST were up-regulated in both shoots and roots under
ABA treatment and various stresses, including salt, drought,
cold and heat stress. The effect of nodules on MsGST gene
expression indicated that the induction of MsGST
expression by abiotic stress is independent of rhizobium
symbiosis. In conclusion, the MsGST gene may be involved
in response to different abiotic stresses in alfalfa.

Keywords: Medicago sativa, cloning, expression, glutathione S-transferase, abiotic stress


Year: 2017
Volume: 77
Issue: 3
Article DOI: 10.5958/0975-6906.2017.00053.0
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Yuman Cao info_circle
Jincai Geng info_circle
Penghui Ren info_circle
Zhang You info_circle
Yafang Wang info_circle
Zhiqiang Zhang info_circle
Jieyu Yang info_circle

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