Indian Society of Genetics & Plant Breeding

Cloning and differential expression analysis of a new rbcS gene from Lemna gibba

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A novel Ribulose-1,5-bisphosphate carboxylase/oxygenase
(Rubisco) small subunit gene (named ssu4d) was cloned
from Lemna gibba by a novel chromosome walking
technology.The full-length of ssu4d cDNA (named ssu4dc),
contained a 522 bp open reading frame encoding a protein
of 174 amino acids. Sequence analysis of ssu4dc and ssu4d
showed that ssu4d contained an intron between +355 nt to
+1125 nt downstream of transcriptional iniative site. ssu4dc
contained 54 bp of 5' untranslated region (UTR), and an
open reading frame of 174 amino acids consisting of a
chloroplast transit peptide with 57 amino acids and a mature
protein of 117 amino acids. The deduced amino acid
sequence of ssu4dc shared 95-96% identity with L. gibba
RbcS protein. Real time-PCR analysis showed differential
expression of individual rbcS genes in light-grown Lemna
gibba. And the levels of SSU4dc mRNA was regulated by
the action of phytochrome, there was variability in the
amount of expression of SSU4dc RNA comprared to the
SSU1 and SSU5B from Lemna gibba.

Keywords: Cloning, new rbcS gene, lemna gibba, transcriptional analysis, differential Expression


Year: 2014
Volume: 74
Issue: 4
Article DOI: 10.5958/0975-6906.2014.00884.0
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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