Indian Society of Genetics & Plant Breeding

Choice of microsatellite markers for isolation of fertility restorers of wild abortive (WA) type cytoplasmic male sterility in rice

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Simple sequence length polymorphism was examined in a
set of eight effective restorers, four partial restorers, two
weak maintainers and four complete maintainers of WA
type CMS system in rice using a set of thirty-two
microsatellite primer pairs in order to assess the nature
and extent of genetic variation among them at molecular
level. Altogether 248 allelic variants were detected with an
average of 7.75 alleles per primer pairs. Polymorphic
information content ranged from 0.508 for RM 6737 to 0.882
for RM 1 with an average value of 0.740 per primer pair.
Amongst the primer pairs used, RM 1, RM 206, RM 591 RM
3873 and RM 8146 had remarkably higher number of allelic
variants with greater gene diversity and genotype
discrimination ability. Using similarity coefficient in
numerical taxonomic approach of classification, the entries
were differentiated and classified into different groups.
Analysis based on molecular profiles allowed discrimination
of effective fertility restorers from rest of the entries
including partial fertility restorers, weak sterility maintainers
and complete sterility maintainers. Principal coordinate
analysis based two dimensional plotting of genetic profiles
completely supported the results obtained from hierarchical
classification of entries and effective fertility restorers were
distinctly discriminated from the remaining entries. Four
microsatellite primer pairs namely, RM 171, RM 315, RM
3873 and RM 6100, which allowed unambiguous
discrimination of the eight effective fertility restorers from
rest of the entries. These microsatellite primer pairs, may
be further utilized in identification of effective fertility
restorers of WA based CMS system in rice.
 

Keywords: Rice, WA, CMS, SSR, PCA, gene diversity, fertility restoration

Info

Year: 2018
Volume: 78
Issue: 2
Article DOI: 10.5958/0975-6906.2018.00026.3
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

info_circle
Rima Kumari info_circle
V. K. Sharma info_circle

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