Characterization of root-knot nematode responsive and root-specific promoter containing PIN domain from Arabidopsis thaliana (L.) Heynh.

Published on May 29, 2018 03:09 AM

Root-knot nematodes (Meloidogyne incognita) are obligate
plant parasites, causing significant economic loss, that
alter expression of host genes in order to establish and
maintain their feeding sites in the roots of host plants. In
the present study, a nematode-responsive-root-specific
gene (AT1G26530) was identified which expressed in roots
of Arabidopsis thaliana after nematode infection.
Quantitative RT-PCR analysis of this gene revealed
maximum (~2.58 fold) up-regulation at 21 days post
inoculation of nematode. A 1580 bp region upstream of
the translational start site of AT1G26530 was isolated and
transformed into Arabidopsis through floral dip method.
On analysis of Arabidopsis transgenic plants harboring
AT1G26530 prm::GUS fusion, reporter gene expression was
seen exclusively in galls after nematode inoculation.
Interestingly, strong GUS activity was observed at early
stages of nematode infection, starting from 14 days and
was sustained up to 30 days post inoculation. Furthermore,
85 to 93 % galls exhibited GUS activity in the nematode
feeding sites. The specificity of the activity of the AT1G26530
promoter, in terms of nematode-responsiveness and rootspecificity, makes it a suitable candidate to express dsRNA
of nematode genes and engineer plants with resistance
against root-knot nematodes using HD-RNAi technology.
 

Keywords: Characterization of root-knot nematode responsive and root-specific promoter containing PIN domain from Arabidopsis thaliana (L.) Heynh.

Info

Year: 2016
Volume: 76
Issue: 1
Article DOI: 10.5958/0975-6906.2016.00011.0
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

Anil Kumar info_circle
Ila Joshi info_circle
Deshika Kohli info_circle
V. Satheesh info_circle
M. Z. Abdin info_circle
Anil Sirohi info_circle
R. Srinivasan info_circle
P. K. Jain info_circle

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