Indian Society of Genetics & Plant Breeding

Characterization of citrus germplasm using simple sequence repeat (SSR) markers

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In the present study, 25 simple sequence repeat markers
were used to detect molecular polymorphism among 30
citrus genotypes representing seedling selections, clonal
selections, exotic and indigenous species. A total of 87
alleles were detected and the banding pattern resolved
by each primer pair was in accordance with single locus
variation. Out of 25 primer pairs, 20 were found
polymorphic and produced alleles ranging between 2 to 6
with an average value of 3.95. The polymorphism
information content ranged from 0.16 for DY263095 to 0.39
for DY289396. Out of 20 SSR markers, 10 SSRs amplified
specific alleles among eight genotypes. The genetic
similarity coefficient for all accessions ranged from 0.11-
0.85%. Cluster analysis separated the genotypes into two
groups at 0.11% similarities. The first major group
comprised four species (G-26, G-27, G-29 and G-30). The
2nd major group consisted of 26 genotypes. Results
confirmed that seedling origin pummelo (G-1 to G-5) are
not true pummelo possibly because of their hybrid origin.
However, seedless clone (G-18) and early bearing clone
(G-20) of Nagpur mandarin showed similarity (78% and
69%, respectively) with Nagpur mandarin. The dwarf cluster
bearing probable citrus hybrid exhibited affinity with sweet
lime and constituted a group with 54% similarity.

Keywords: Citrus, germplasm, molecular charachterization, simple sequence repeat markers


Year: 2011
Volume: 71
Issue: 4
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


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