Indian Society of Genetics & Plant Breeding

Important

Callus induction and whole plant regeneration in sub-tropical maize (Zea mays L.) using mature embryos as explants

Published on

Thirteen sub-tropical maize genotypes were evaluated for
in vitro callus induction and whole plant regeneration. Seeds
were germinated on MS-based medium supplemented with
10 mg l–1 picloram and 3.0 mg l–1 BAP which induced the
formation of nodes. The developed nodes were excised
about 0.25 cm above and below and were split longitudinally
into two halves. The split nodes were placed on the callusing
medium supplemented with 2.2 mg l–1 picloram and 0.5
mg l–1 2, 4-D, the cut surface facing down and touching the
media. Among the thirteen genotypes, embryogenic calli
were induced in seven genotypes ranging between 10.78-
65.65%. Out of seven genotypes, only one genotype i.e.,
VQL 2 regenerated in to a whole plant with a frequency of
34% on MS basal medium under 80 μM m–2 s–1 of light
intensity. Regeneration medium and light intensity played
key role for the initiation of somatic embryogenesis and
regeneration of whole plants. Survival efficiency of in vitro
regenerated plantlets was found to be 95% out of which
85% of the plants grew normally without any morphological
abnormality.

Keywords: Maize, mature embryo, regeneration, VQL2

Info

Year: 2015
Volume: 75
Issue: 3
Article DOI: 10.5958/0975-6906.2015.00052.8
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

info_circle
P. K. Agrawal info_circle
V. Pande info_circle
H. S. Gupta info_circle

Download PDF