Indian Society of Genetics & Plant Breeding

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Breeding of black gram genotypes for resistance to bruchid beetle through molecular and conventional approaches

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Vigna mungo var. silvestris (Trombay wild urd                
(INGR10133)) collected from Trombay hilly areas has been     
shown to have bruchid resistance. Recombinant inbred         

lines (RILs) were generated by crossing V. mungo var.        

mungo (cv. TU 94-2, bruchid susceptible) and V mungo         
var. silvestris (bruchid resistant). A genetic linkage map 
                                                             
constructed using 104 recombinant inbred line (RIL) in F9 
generation with 428 markers (86 RAPD, 47 SSR, 41 ISSR        
and 254 AFLP) was used for quantitative trait loci (QTL)     

detection. The RILs exhibited a high level of variation for  

percentage of adult emergence (0-100%) and                  
developmental period (0-105 days). Two QTLs Cmrae1.1         
and Cmrae1.2 were identified for percentage of adult 
emergence on the linkage group (LG) 3 and 4 respectively.    
For developmental period, six QTLs were identified with     
two QTLs (Cmrdp1.1 and Cmrdp1.2) on LG 1, three QTLs 

(Cmrdp1.3 Cmrdp 1.4 and Cmrdp1.5) on LG 2 and one QTL        
(Cmrdpl.6) on LG 10. DNA markers linked to bruchid           
resistance will aid in marker assisted selection for transfer 
of this trait into different genetic background. Selections  
from the cross between TU 94-2 and V mungo var. silvestris   
has led to the development of new black gram genotypes       

TU-26,TU-40 and TU-72 with resistance to bruchids, which     
are under different stages of testing.                

Keywords: Antibiosis, Callosobruchus maculatus, DNA markers, Vigna mungo, Quantitative trait loci

Info

Year: 2011
Volume: 71
Issue: 2
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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