Indian Society of Genetics & Plant Breeding

Biochemical screening for trypsin inhibitor factors and morphomolecular characterization of soybean (Glycine max L. Merr.)

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A set of 101 diverse soybean genotypes were biochemically
analyzed for trypsin inhibitor (TI) contents, and were
characterized with morphological and molecular markers.
The TI content in the seeds ranged from 14.65 to 175.52 mg
g–1 soymeal. The Indian soybean varieties were found to
contain higher amount of TI (58.79-126.78 mg g–1 soymeal)
while PI542044, an exotic germplasm was found to contain
the least (14.65 mg g–1 soymeal). Morphological
characterization with 16 qualitative and quantitative traits
revealed significant variations and grouped the genotypes
in to three major groups with overall mean dissimilarity
value of 0.30. Genetic diversity study conducted with 100
simple sequence repeat (SSR) markers detected higher level
of polymorphism (71%). Of the total 221 alleles amplified,
65 were rare (frequency <0.2). The mean polymorphism
information content (PIC) of the markers was 0.27 while the
value for gene diversity was 0.44. UPGMA-based cluster
analysis grouped the genotypes into four major clusters of
which I, II and III were occupied by genotypes containing
higher and moderate level of TI. PI542044, which was free
from Kunitz trypsin inhibitor (KTI), grouped singly. Such
genotype would be suitable for use in breeding program
directed towards development of varieties with zero KTI.

Keywords: sequence repeats, morphological diversity, genetic diversity


Year: 2015
Volume: 75
Issue: 4
Article DOI: 10.5958/0975-6906.2015.00078.4
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Akshay Talukdar,S. K. Lal,R. L. Sapra, G. D. Haris info_circle
M. Shivakumar info_circle
Bhupender Kumar info_circle
Vanishree Girmilla info_circle

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