Indian Society of Genetics & Plant Breeding

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Biochemical and molecular markers for establishing distinctiveness of aromatic rice (Oryza sativa L.) varieties

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Use of biochemical and molecular markers in DUS testing
for establishing distinctiveness as a complement to
morphological descriptors has been attempted in this
study. Twenty indigenous aromatic rice (Oryza sativa L.)
varieties were studied for morphological descriptors, total
soluble proteins and isozymes as biochemical and RAPD
molecular markers for determining distinctive features.
SDS-PAGE for total soluble proteins and isozyme analysis
revealed moderate and moderate to high degree of
polymorphism respectively. UPGMA analysis of combined
isozyme data of different enzymes could discriminate all
varieties except Bindli from Tilakchandan and two related
varieties of Tilakchandan. In general neither morphological
descriptors nor biochemical markers could discriminate
especially related indigenous varieties of a particular
group. A high degree of polymorphism was detected
among the twenty aromatic rice varieties through 9 random
primers used for RAPD marker analysis. UPGMA cluster
analysis of RAPD data could distinguish all the twenty
rice varieties. It can be concluded that in situations where
the morpho-physiological DUS descriptors are not able
to establish distinctiveness of a variety then biochemical
and molecular markers may be used as additional or
complement descriptors for resolving distinctiveness of
indigenous related varieties.
 

Keywords: Rice, DUS, isozymes, RAPD

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Year: 2010
Volume: 70
Issue: 1
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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