Indian Society of Genetics & Plant Breeding

Assessment of somaclonal variations in two lines of pearl millet [Pennisetum glaucum (L.) R. Sr.]

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Somaclonal variation has great potential to enhance the
advantages of conventional plant breeding, and increase
the production and productivity of crops to meet the
increasing demand for food and food products in the
future. In pearl millet [Pennisetum glaucum (L.) R. Sr.],
plant morphogenesis has been achieved via somatic
embryogenesis. Somatic embryos were isolated and
regenerated into whole green plants on MS medium
supplemented with indole-3-acetic acid (1 mgl-1) and
kinetin (0.5 mgl-1). Ten Ro plants of each cultivar were
selected on the basis of 13 quantitative characters.
Transmission of somaclonal variation was studied from
callus derived (Ro) plants to R1 and R2 generations and
were compared with those obtained from embryo culture
without any callus formation (Eo-E2). Significant variation
was observed among regenerants for number of leaves!
plant, days to heading,. length of panicle, average width
of panicle and weight of panicle.

Keywords: Pearl millet, agronomic performance, somatic embryogenesis, tissue culture


Year: 2003
Volume: 63
Issue: 4
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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