Indian Society of Genetics & Plant Breeding

Assessment of genetic diversity of sesame (Sesamum indicum L.) genotypes using morphological and RAPD markers

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A comparison was made between phenotypic and
molecular markers (RAPD) to assess the genetic diversity
of twenty sesame genotypes representing, different agroecological zones of India. A higher range of genetic
similarity (0.88-0.99) was observed on the basis of 20
phenotypic markers whereas, it was less (0.78-0.95) on
the basis of RAPD markers, indicating better distinction
between and within population of sesame when RAPD
markers were used. Genotypes in RAPD based
dendrogram were clustered into their respective
geographical group while, a random grouping was
observed in dendrogram based on the morphological
markers. The genotypic classification agreed closely with
the grouping observed in RAPD based principal coordinate
analysis (PCA). The mean coefficient of genetic
differentiation (Gst 0.619) indicated a high level of genetic
diversity among population (Ht 0.169) and low level of
genetic diversity within population (Hs 0.048). A negative
correlation was found among discrimination power (Dj)
and polymorphism information content (PIC) but the
former was found effective in distinguishing all the
populations as well as genotypes individually using
specific band positions for them.

Keywords: Sesame, genetic diversity, morphological markers, RAPD marker


Year: 2009
Volume: 69
Issue: 3
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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