Indian Society of Genetics & Plant Breeding

Assessment of genetic diversity of rainfed lowland rice genotypes using microsatellite markers

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Genetic diversity in rainfed lowland rice genotypes
constituting landraces, high yielding varieties and improved
lines was assessed using microsatellite markers distributed
over 12 chromosomes of rice. A total of 143 alleles were
amplified, of which 136 were polymorphic. The
polymorphism information content (PIC) and discriminating
power of microsatellite markers ranged between 0 and 0.91
with an average of 0.66 per locus. Genetic similarities among
genotypes varied from 0.37 to 0.89 with an average of 0.6.
The UPGMA cluster analysis grouped the rice genotypes
into two major clusters at 50% level of genetic similarity.All
the genotypes studied could be uniquely distinguished
from each other at probability of 2.2 x 10–13 of identical
match by chance. The high level of genetic variability was
detected among the lowland rice genotypes which would
enable for selection of diverse donors in the development
high yielding rice varieties suitable for rainfed lowland
ecosystem. Seven genotype with specific unique alleles
were identified, which can be used as molecular tags. A
basic molecular database was created for rainfed lowland
genotypes which will be useful for future reference and to
protect this unique rice under IPR regime.

Keywords: Genetic diversity, low land rice, microsatellite marker

Info

Year: 2013
Volume: 73
Issue: 2
 Article DOI: 10.5958/j.0975-6906.73.2.021
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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